hffc  (CLS Cell Lines Service GmbH)

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: HFFC diet promotes weight gain and MASH. Mice fed control diet or HFFC diet for 24 weeks. ( A , B ) % change in relative body weight and % liver-to-body weight ratio ( A ) and levels of triglyceride and cholesterol in liver ( B ), comparing diets (n = 11–17). ( C ) Liver qPCR for indicated gene expression, normalized by 36b4 (n = 11–15). ( D ) Liver sections were stained with hematoxylin and eosin (H&E) or underwent immunohistochemistry (IHC) with antibody-detecting ki67 or p62, with scale in panel. ( E ) Steatosis and inflammation in liver determined using H&E-stained sections, and % of ki67 positive cells and % area of p62 determined using IHC (n = 8–17). Data in ( A – C , E ) are mean ± standard error of the mean, with individual data points shown. p -value was determined by multiple unpaired t -tests, with adjustment for multiple comparisons. In ( D ), black arrows indicate Ki67 + nuclei and blue arrows indicate p62 + area.

Article Snippet: Mice were fed a control diet containing 10% fat (lard and soybean oil), 10% sucrose, 0% fructose, and 0% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D09100304) or MASH-inducing HFFC diet containing 40% fat (75% palm oil/11% lard/14% soybean oil), 10% sucrose, 20% fructose and 1% or 2% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D19021910 or D09100310), as indicated in the text and figures.

Techniques: Control, Gene Expression, Staining, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: Effect of hepatocyte deficiency for Nrf1, Nrf2, or both in mice chronically fed HFFC diet. Mice fed HFFC diet for 24 weeks. In ( A – C ), mice were infected with indicated virus on week 22. In ( D ), mice were infected on week 16. ( A ) Liver sections stained with hematoxylin and eosin, with scale indicated, and steatosis and inflammation (n = 5–14). ( B ) Levels of triglyceride and cholesterol in liver (n = 6–14). ( C ) qPCR analysis for indicated gene expression, normalized by 36b4 (n = 5–14). ( D ) Representative sections for immunohistochemistry (IHC) with antibody-detecting ki67 or p62, with scale indicated, and % of ki67 positive cells and % area of p62 (n = 6–23). Data are mean ± standard error of the mean, with individual data points shown (males = circles; females = triangle). In ( A – C ), p -value was determined by two-way analysis of variance, with Sidak post-test. In ( D ), p -value was determined by one-way analysis of variance, with Dunnett post-test. In ( D ), black arrows indicate Ki67 + nuclei and blue arrows indicate p62 + area.

Article Snippet: Mice were fed a control diet containing 10% fat (lard and soybean oil), 10% sucrose, 0% fructose, and 0% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D09100304) or MASH-inducing HFFC diet containing 40% fat (75% palm oil/11% lard/14% soybean oil), 10% sucrose, 20% fructose and 1% or 2% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D19021910 or D09100310), as indicated in the text and figures.

Techniques: Infection, Virus, Staining, Gene Expression, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: Effect of hepatocyte deficiency for Nrf1, Nrf2, or both on liver tumor development. ( A ) Study design showing that mice were fed HFFC diet with 2% cholesterol for 52 weeks and infected on weeks 16, 32, and 48 with indicated virus. Liver tumor analysis was performed at the endpoint. ( B , C ) % incidence of liver tumors ( B ) and the number of tumors per liver ( C ) in males (n = 5–7) and females (n = 5–6). ( D ) Volume of largest liver tumor in males (n = 5–7) and females (n = 5–6) and representative tumor images (black arrows indicate tumors). Data are mean ± standard error of the mean, with individual data points shown. The p -value was determined by two-way analysis of variance, with Sidak post-test.

Article Snippet: Mice were fed a control diet containing 10% fat (lard and soybean oil), 10% sucrose, 0% fructose, and 0% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D09100304) or MASH-inducing HFFC diet containing 40% fat (75% palm oil/11% lard/14% soybean oil), 10% sucrose, 20% fructose and 1% or 2% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D19021910 or D09100310), as indicated in the text and figures.

Techniques: Infection, Virus

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: Combined effect of Nrf2-inducing drug bardoxolone and Nrf1 overexpression on MASH-linked fibrosis. ( A ) Study design showing C57bl/6J mice that were fed HFFC diet with 2% cholesterol for 24 weeks. Mice were injected with carbon tetrachloride once per week from week 0–15 to induce liver fibrosis. On weeks 16–24, mice were treated as indicated with modulators of Nrf1 and Nrf2 activity. Liver analysis was performed at the endpoint. ( B ) Liver sections stained with hematoxylin and eosin, with scale indicated in panel, and corresponding score for steatosis and inflammation in liver (n = 12–15). ( C ) Liver sections stained with fibrosis detecting sirius red, with scale indicated in panel, and corresponding % sirius red + area (n = 10–14). ( D ) Liver qPCR analysis for indicated gene expression, normalized by ribosomal protein 36b4 (n = 11–15). Data are mean ± standard error of the mean, with individual data points shown (males = circles; females = triangles). The p -value was determined by one-way analysis of variance, with Dunnett post-test.

Article Snippet: Mice were fed a control diet containing 10% fat (lard and soybean oil), 10% sucrose, 0% fructose, and 0% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D09100304) or MASH-inducing HFFC diet containing 40% fat (75% palm oil/11% lard/14% soybean oil), 10% sucrose, 20% fructose and 1% or 2% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D19021910 or D09100310), as indicated in the text and figures.

Techniques: Over Expression, Injection, Activity Assay, Staining, Gene Expression